Transformable Thymine-Requiring Mutant of Bacillus subtilis
نویسندگان
چکیده
منابع مشابه
Uracil incorporation into nascent DNA of thymine-requiring mutant of Bacillus subtilis 168.
A thymine-requiring mutant of Bacillus subtilis strain 168 accumulates short DNA chains after brief pulses with [(3)H]thymidine. Reversion of the thy mutation to thy(+) abolishes the accumulation of short DNA chains, suggesting that the accumulation is related to the thy mutation. The reason for this accumulation has been further investigated by analysis of a mutant with a defective uracil-DNA ...
متن کاملConversion of uracil to thymine by strains of bacillus subtilis.
From replacement studies (1) and inhibition analysis (2) with microorganisms requiring folic acid, it has been inferred that this vitamin is concerned in the biosynthesis of purines and of thymine. More directly, folic acid has been shown to be involved in nucleic acid synthesis in Lactobacillus casei (3,4). According to Buchanan and Schulman (5), the formation of purine derivatives would seem ...
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An ethidium bromide-resistant mutant (EB8) derived from a Marburg strain of Bacillus subtilis was found to be conditionally resistant to 10 mug of ethidium bromide per ml. Expression of resistance is complete only during vegetative growth at incubation temperatures above 30 C in complex medium or minimal medium supplemented with Casamino Acids. Strain EB8 is cross-resistant to acriflavine and p...
متن کاملPhospholipid metabolism in the absence of net phospholipid synthesis in a glycerol-requiring mutant of Bacillus subtilis.
A glycerol-requiring auxotroph of Bacillus subtilis showed no net synthesis of phospholipid when deprived of glycerol. Although there was no net synthesis of phospholipid, we found that: (i) fatty acids and (32)P were slowly incorporated into phospholipid; (ii) in pulse-chase experiments, both (32)P and (14)C in the glycerol portion of the phospholipids were lost from phosphatidlyglycerol (PG) ...
متن کاملGenome Sequence of the Bacillus subtilis Biofilm-Forming Transformable Strain PS216
Bacillus subtilis PS216, a strain isolated in Slovenia, has been sequenced. PS216 is transformable and forms robust biofilms, making it useful for the study of competence regulation in an undomesticated bacterium.
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1965
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.89.1.262-263.1965